Is there any significant disadvantage in preparing a protein shake 12 hours before consuming it, or should it be consumed as soon as it has been mixed with water or milk as it will degrade quickly? Thinking about preparing it in the morning and then taking it with me to work and drink it in the afternoon.
I asked one protein powder manufacturer (Myprotein) and they simply responded that they recommend to consume it directly after mixing, but not if or why waiting would be a problem.
It wasn’t a scientist but someone from customer service. In the same e-mail, I also asked them if casein or whey degrades when mixing it with hot milk, to which they replied “Using milk is not as effective as using water with the product.”
Also, a protein rich solution is an ideal environment for bacterial growth, especially if you facilitate formation of low oxygen conditions with a sealed container. Granted, 12 hours is probably not long enough for it to spoil, even at room temperature, but I would say you should keep it either refrigerated or cold in a thermos just to be on the safe side.
Eh, I have no problem with recommending caution about potential spoilage, but I would say whey or “protein-rich” solutions are ideal for bacterial growth. Needs more sugar.